Article
Ser57 in the Na+/proline permease of Escherichia coli is critical for high-affinity proline uptake.
European journal of biochemistry - 1 Aug 1996
Quick M, Tebbe S, Jung H
Abstract excerpt
Ser57 in the Na+/proline permease of Escherichia coli has been replaced with alanine, cysteine, glycine, or threonine, and properties of the corresponding putP mutants have been analyzed. Although Ser57 is not essential for activity, the amino acid side chain at this position is critical for prol...
Topics
- Amino Acid Sequence
- Amino Acid Transport Systems, Neutral
- Binding Sites
- Biological Transport
- Cell Compartmentation
- Escherichia coli
- Genetic Complementation Test
- Kinetics
- Membrane Transport Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Proline
- Sequence Analysis, DNA
- Serine