Article
Altered ionic selectivity of the sodium channel revealed by cysteine mutations within the pore.
The Journal of general physiology - 1 Apr 1997
Tsushima R G, Li R A, Backx P H
Abstract excerpt
To explore the role of pore-lining amino acids in Na+ channel ion-selectivity, pore residues were replaced serially with cysteine in cloned rat skeletal muscle Na+ channels. Ionic selectivity was determined by measuring permeability and ionic current ratios of whole-cell currents in Xenopus oocyt...
Topics
- Animals
- Cations, Divalent
- Cell Membrane Permeability
- Cysteine
- Electric Stimulation
- Electrophysiology
- Female
- Hydrogen-Ion Concentration
- Membrane Potentials
- Muscle, Skeletal
- Mutagenesis, Site-Directed
- Mutation
- Oocytes
- Patch-Clamp Techniques
- Rats
- Sodium Channels
- Sulfhydryl Compounds
- Xenopus laevis