Structural determinants of the uridine-preferring specificity of RNase PL3.

doi:10.1021/bi960457e

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Article

Structural determinants of the uridine-preferring specificity of RNase PL3.

Biochemistry - 16 Jul 1996

Vicentini A M, Kote-Jarai Z, Hofsteenge J

Abstract excerpt

RNase PL3 is a structurally highly conserved, pyrimidine-specific RNase, which strongly prefers to cleave at the 3'-side of uridine. Here, question of which residues are involved in determining substrate specificity is addressed. The difference in the rate of cleavage of UpA and CpA was found to...

Topics

Animals
Binding Sites
Cytidine
Dinucleoside Phosphates
Endoribonucleases
Escherichia coli
Hydrogen Bonding
Kinetics
Liver
Models, Molecular
Molecular Structure
Mutagenesis, Site-Directed
Mutation
Poly C
Poly U
Recombinant Proteins
Substrate Specificity
Swine

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