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Article

The catalytic activity of cytochrome P450cam towards styrene oxidation is increased by site-specific mutagenesis.

FEBS letters - 24 Mar 1997

Nickerson D P, Harford-Cross C F, Fulcher S R, Wong L L

Abstract excerpt

The styrene oxidation activity of cytochrome P450cam, has been greatly improved by rational protein engineering. Compared to the wild-type enzyme, the active-site mutants Y96A and Y96F bound styrene more tightly, consumed NADH more rapidly, and were more efficient at utilising reducing equivalents for product formation. Styrene oxide formation rates were enhanced 9-fold in the Y96A mutant relative to wild-type,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 9089281
DOI: 10.1016/s0014-5793(97)00174-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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