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Article

Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73.

Journal of bacteriology - 1 Jul 1998

Kim K, Lee S, Lee K, Lim D

Abstract excerpt

To understand the mechanism underlying toluene resistance of a toluene-tolerant bacterium, Pseudomonas putida GM73, we carried out Tn5 mutagenesis and isolated eight toluene-sensitive mutants. None of the mutants grew in the presence of 20% (vol/vol) toluene in growth medium but exhibited differential sensitivity to toluene. When wild-type cells were treated with toluene (1% [vol/vol]) for 5 min, about 2% of the...

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Identifiers

PMID: 9658016
DOI: 10.1128/JB.180.14.3692-3696.1998
PMCID: PMC107341

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