Article
Isolation and characterization of toluene-sensitive mutants from Pseudomonas putida IH-2000.
FEMS microbiology letters - 15 Dec 1998
Hirayama H, Takami H, Inoue A, Horikoshi K
Abstract excerpt
Two toluene-sensitive mutants were generated from Pseudomonas putida IH-2000, the first known toluene-tolerant isolate, by Tn5 transposon mutagenesis. These mutants were unable to grow in the presence of toluene (log P(ow) 2.8) but they could grow in medium overlaid with organic solvents having a log P(ow) value higher than that of toluene such as p-xylene (log P(ow) 3.1), cyclohexane (log P(ow) 3.4) and n-hexane...
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