Cloning and expression of the ponB gene, encoding penicillin-binding protein 1B of Escherichia coli, in heterologous systems.

doi:10.1128/jb.172.8.4448-4455.1990

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Article

Cloning and expression of the ponB gene, encoding penicillin-binding protein 1B of Escherichia coli, in heterologous systems.

Journal of bacteriology - 1 Aug 1990

Plá J, Rojo F, de Pedro M A, Ayala J A

Abstract excerpt

A fragment from the ponB region of the Escherichia coli chromosome comprising the promoterless sequence encoding penicillin-binding protein 1B (PBP 1B) has been cloned in a broad-host-range expression vector under the control of the kanamycin resistance gene promoter present in the vector. The hybrid plasmid (pJP3) was used to transform appropriate strains of Salmonella typhimurium, Pseudomonas putida, and...

Topics

Ampicillin
Bacterial Proteins
Carrier Proteins
Cell Membrane
Cloning, Molecular
Escherichia coli
Gene Expression
Genes, Bacterial
Genotype
Hexosyltransferases
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microscopy, Electron

Muramoylpentapeptide Carboxypeptidase

Penicillin-Binding Proteins

Penicillins

Peptidyl Transferases

Plasmids

Pseudomonas

Source

Open in PubMed Open full text

Identifiers

PMID: 2198260
DOI: 10.1128/jb.172.8.4448-4455.1990
PMCID: PMC213274

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2198260-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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