Article
Multiplex mutagenically separated PCR: diagnosis of beta-thalassemia and hemoglobin variants.
BioTechniques - 1 Mar 1997
Chang J G, Liu H J, Huang J M, Yang T Y, Chang C P
Abstract excerpt
A rapid and simple method, termed multiplex mutagenically separated PCR (MS-PCR), was developed to detect several molecular defects in the hemoglobin gene in one PCR. This technique, in which different-size allele-specific primers were used, specifically amplified both normal and mutant alleles of the globin gene in the same reaction. Subsequent gel electrophoresis showed at least one of the two allelic products...
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