Article
Multiplex mutagenically separated PCR: diagnosis of beta-thalassemia and hemoglobin variants.
BioTechniques - 1 Mar 1997
Chang J G, Liu H J, Huang J M, Yang T Y, Chang C P
Abstract excerpt
A rapid and simple method, termed multiplex mutagenically separated PCR (MS-PCR), was developed to detect several molecular defects in the hemoglobin gene in one PCR. This technique, in which different-size allele-specific primers were used, specifically amplified both normal and mutant alleles o...
Topics
- Alleles
- Asian People
- DNA Primers
- Electrophoresis, Agar Gel
- Globins
- Hemoglobins
- Heterozygote
- Humans
- Mutation
- Polymerase Chain Reaction
- Taiwan
- beta-Thalassemia