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[Multiplexed mutagenically separated PCR assay for rapid detection of SNP loci in mitochondrial DNA coding region].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Feb 2007

Ran Peng, Li Ying-bi, Yan Jing, Zhang Bei-lei, Zhang Ji, Shan Hua-xin, Ye Zhi-peng, Hou Yi-ping, Zhang Lin, Liao Miao, Wu Jin

Abstract excerpt

OBJECTIVE: To develop a multiplexed mutagenically separated PCR (MS-PCR) for single nucleotide polymorphism (SNP) loci typing in mitochondrial DNA coding regions and to study the applications in investigating the allele frequencies and haplotypes of four SNP loci in mitochondrial DNA coding regions in Chinese Chengdu Han population. METHODS: Four SNP loci C12705T, A8701G, G8584A and C10400T, two allele specific...

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PMID: 17285547

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