Article
Rapid detection of hemoglobin variants by mutagenically separated polymerase chain reaction (MS-PCR).
Annals of hematology - 1 Aug 1995
Chang J G, Chang C P, Lu C M, Huang J M, Chen J T, Liu H J
Abstract excerpt
The detection of molecular defects of hemoglobin variants using mutagenically separated polymerase chain reaction (MS-PCR) was applied in this study. Using different lengths of allele-specific mutagenic primers, normal and mutant alleles of hemoglobin genes were amplified in the same reaction tube. Subsequent gel electrophoresis showed at least one of the two allelic products at the same loci or at least two of...
Topics
- Alleles
- Base Sequence
- DNA Primers
- Hemoglobin E
- Hemoglobins, Abnormal
- Humans
- Molecular Sequence Data
- Mutagenesis
- Polymerase Chain Reaction