Article
Recombinant strategies for rapid purification of catalytic subunits of cAMP-dependent protein kinase.
Analytical biochemistry - 15 Feb 1997
Hemmer W, McGlone M, Taylor S S
Abstract excerpt
Knowledge of the crystal structure of the catalytic subunit (C) of cAMP-dependent protein kinase provided for the first time a molecular basis for probing function by site-directed mutagenesis. The purification of mutant C-subunits, however, presented new and unanticipated challenges due to insta...
Topics
- Adenosine Triphosphate
- Binding Sites
- Cyclic AMP
- Cyclic AMP-Dependent Protein Kinase Type II
- Cyclic AMP-Dependent Protein Kinases
- Electrophoresis
- Enzyme Activation
- Isoelectric Focusing
- Isoenzymes
- Kinetics
- Mutation
- Phosphorylation
- Recombinant Proteins