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Article

Recombinant strategies for rapid purification of catalytic subunits of cAMP-dependent protein kinase.

Analytical biochemistry - 15 Feb 1997

Hemmer W, McGlone M, Taylor S S

Abstract excerpt

Knowledge of the crystal structure of the catalytic subunit (C) of cAMP-dependent protein kinase provided for the first time a molecular basis for probing function by site-directed mutagenesis. The purification of mutant C-subunits, however, presented new and unanticipated challenges due to instability, insolubility, and underphosphorylation of the altered proteins. To overcome these barriers, a rapid and...

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Identifiers

PMID: 9056191
DOI: 10.1006/abio.1996.9952

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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