Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase.

doi:10.1021/bi9620215

Back to search

Article

Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase.

Biochemistry - 25 Feb 1997

Lawson S L, Wakarchuk W W, Withers S G

Abstract excerpt

The mechanism of action employed by a glycosidase is dictated, in part, by the distance between the two catalytic carboxylic acids. In the retaining endo-beta-1,4-xylanase from Bacillus circulans, this critical distance (approximately 5.5 A) has been altered by mutagenesis of the putative acid/ba...

Topics

Bacillus
Enzyme Activation
Glutamic Acid
Mutation
Xylan Endo-1,3-beta-Xylosidase
Xylosidases

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.