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Article

Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase.

Biochemistry - 25 Feb 1997

Lawson S L, Wakarchuk W W, Withers S G

Abstract excerpt

The mechanism of action employed by a glycosidase is dictated, in part, by the distance between the two catalytic carboxylic acids. In the retaining endo-beta-1,4-xylanase from Bacillus circulans, this critical distance (approximately 5.5 A) has been altered by mutagenesis of the putative acid/base catalyst Glu172. An increase in the separation (Glu172Asp) resulted in a 400-fold decrease in the k(cat) value for...

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PMID: 9047328
DOI: 10.1021/bi9620215

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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