Article
The lipid A biosynthesis deficiency of the Escherichia coli antibiotic-supersensitive mutant LH530 is suppressed by a novel locus, ORF195.
Microbiology (Reading, England) - 1 Jan 1997
Hirvas Laura, Nurminen Marjatta, Helander Ilkka M, Vuorio Riitta, Vaara Martti
Abstract excerpt
A new mutant of Escherichia coli K-12 supersensitive to both hydrophobic and large hydrophilic antibiotics was isolated and characterized. The mutant grew well at 28 degrees C, poorly at 37 degrees C, and did not grow at 42 degrees C. The rate of its lipid A biosynthesis was reduced as compared t...
Topics
- Anti-Bacterial Agents
- Cell Membrane Permeability
- Chromosome Mapping
- Cloning, Molecular
- Escherichia coli
- Gene Dosage
- Genes, Bacterial
- Genetic Complementation Test
- Lipid A
- Lipopolysaccharides
- Microbial Sensitivity Tests
- Mutagenesis
- Open Reading Frames
- Phenotype
- Phospholipids
- Sequence Analysis, DNA
- Suppression, Genetic
- Temperature