Article
An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
Development (Cambridge, England) - 1 Dec 1996
Zernicka-Goetz M, Pines J, Ryan K, Siemering K R, Haseloff J, Evans M J, Gurdon J B
Abstract excerpt
We describe the use of a DNA construct (named GFP.RN3) encoding green fluorescent protein as a lineage marker for Xenopus embryos. This offers the following advantages over other lineage markers so far used in Xenopus. When injected as synthetic mRNA, its protein emits intense fluorescence in liv...
Topics
- Animals
- Biomarkers
- Blastocyst
- Cell Lineage
- Cell Transplantation
- Embryology
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins
- Luminescent Proteins
- Muscles
- Mutation
- T-Box Domain Proteins
- Transcription Factors
- Transcriptional Activation
- Xenopus
- Xenopus Proteins