Article
Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice.
Genesis (New York, N.Y. : 2000) - 1 Nov 2013
Vacaru Andrei M, Isern Joan, Fraser Stuart T, Baron Margaret H
Abstract excerpt
Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to...
Topics
- Animals
- Cell Lineage
- Cell Nucleus
- Cell Proliferation
- Embryo, Mammalian
- Erythroblasts
- Erythropoiesis
- Genes, Reporter
- Genotype
- Green Fluorescent Proteins
- Histones
- Mice
- Mice, Transgenic
- Phagocytosis
- Recombinant Fusion Proteins
- epsilon-Globins