Article
Initial characterization of autoprocessing and active-center mutants of CMV proteinase.
Journal of protein chemistry - 1 Nov 1996
Snyder S W, Edalji R P, Lindh F G, Walter K A, Solomon L, Pratt S, Steffy K, Holzman T F
Abstract excerpt
Human cytomegalovirus (CMV) encodes a unique serine proteinase that is required in the maturation of the viral capsid. The CMV proteinase can undergo autocatalytic activation and is subject to proteolytic self-inactivation. Mutant enzyme forms were prepared to eliminate the initial autoprocessing...
Topics
- Amino Acid Sequence
- Binding Sites
- Calorimetry, Differential Scanning
- Cell Division
- Circular Dichroism
- Cloning, Molecular
- Cytomegalovirus
- Enzyme Stability
- Escherichia coli
- Gene Expression
- Humans
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Folding
- Protein Structure, Secondary
- Recombinant Proteins
- Serine Endopeptidases