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Article

Relationship between opacity of transformed E. coli colonies and over-expression of the recombinant transcript.

BioTechniques - 1 Jan 1997

Barik S

Abstract excerpt

Following transformation with recombinant plasmid clones, E. coli BL21(DE3) often produced extremely opaque colonies on a standard semisolid agar plate, as compared with the translucent colonies produced by normal, untransformed bacteria A standard BL21(DE3) culture consisted of two kinds of cells one kind produced translucent colonies and the other produced opaque colonies upon transformation by recombinant...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 8994658
DOI: 10.2144/97221st03

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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