Article
A dominant negative effect of eth-1r, a mutant allele of the Neurospora crassa S-adenosylmethionine synthetase-encoding gene conferring resistance to the methionine toxic analogue ethionine.
Genetics - 1 Dec 1996
Barra J L, Mautino M R, Rosa A L
Abstract excerpt
eth-1r, a thermosensitive allele of the Neurospora crassa S-adenosylmethionine (AdoMet) synthetase gene that confers ethionine resistance, has been cloned and sequenced. Replacement of an aspartic amino acid residue (D48-->N48), perfectly conserved in prokaryotic, fungal and higher eukaryotic AdoMet synthetases, was found responsible for both thermosensitivity and ethionine resistance conferred by eth-1r. Gene...
Topics
- Alleles
- Amino Acid Sequence
- Carcinogens
- Drug Resistance, Microbial
- Ethionine
- Methionine
- Methionine Adenosyltransferase
- Molecular Sequence Data
- Mutation
- Neurospora crassa