Article
A robust strategy for screening and confirmation of familial defective apolipoprotein B-100.
Clinical chemistry - 1 Jan 1993
Mamotte C D, van Bockxmeer F M
Abstract excerpt
The diagnosis of familial defective apolipoprotein B-100 (FDB) has been facilitated by the use of mutagenic polymerase chain reaction (PCR) primers to introduce restriction sites at the FDB gene locus. We describe a two-test strategy for diagnosing FDB that overcomes the potential for error in single-test methods based on such techniques. We introduce an Sau96I restriction site for PCR products of the normal...
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