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Article

Guided evolution of enzymes with new substrate specificities.

Journal of molecular biology - 22 Nov 1996

el Hawrani A S, Sessions R B, Moreton K M, Holbrook J J

Abstract excerpt

A gene library was constructed coding for all possible variants of two amino acids (101, 102) in a solvent-exposed surface return loop (alpha E-beta D) of Bacillus stearothermophilus L-lactate dehydrogenase (bsLDH). All but one of 38 enzyme variants examined were thermally stable and had native-like hydrodynamic properties. In this sample, there was no bias detected in either the DNA or amino acid sequences...

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Identifiers

PMID: 8950270
DOI: 10.1006/jmbi.1996.0626

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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