Article
Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli.
Structure (London, England : 1993) - 10 Jan 2001
Wymer N, Buchanan L V, Henderson D, Mehta N, Botting C H, Pocivavsek L, Fierke C A, Toone E J, Naismith J H
Abstract excerpt
BACKGROUND: Aldolases are carbon bond-forming enzymes that have long been identified as useful tools for the organic chemist. However, their utility is limited in part by their narrow substrate utilization. Site-directed mutagenesis of various enzymes to alter their specificity has been performed for many years, typically without the desired effect. More recently directed evolution has been employed to engineer...
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