Article
A protease complex in the Escherichia coli plasma membrane: HflKC (HflA) forms a complex with FtsH (HflB), regulating its proteolytic activity against SecY.
The EMBO journal - 15 Nov 1996
Kihara A, Akiyama Y, Ito K
Abstract excerpt
Escherichia coli FtsH (HflB), a membrane-bound ATPase is required for proteolytic degradation of uncomplexed forms of the protein translocase SecY subunit. We have now isolated SecY-stabilizing mutations that cause an amino acid substitution in the HflK-HflC membrane protein complex. Although Hfl...
Topics
- ATP-Dependent Proteases
- Bacterial Proteins
- Cell Membrane
- Chromatography, Affinity
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Membrane Proteins
- Mutation
- Phenotype
- Precipitin Tests
- SEC Translocation Channels
- Sequence Deletion
- Serine Endopeptidases