Article
Location of the active site and proposed catalytic mechanism of pterin-4a-carbinolamine dehydratase.
European journal of biochemistry - 1 Nov 1996
Köster S, Stier G, Ficner R, Hölzer M, Curtius H C, Suck D, Ghisla S
Abstract excerpt
Based on the recently solved three-dimensional structure of pterin-4a-carbinolamine dehydratase from rat/human liver the involvement of the proposed active-site residues Glu57, Asp60, His61, His62, Tyr69, His79, Arg87 and Asp88 was examined by site-directed mutagenesis. Most of the mutants showed...
Topics
- Amino Acid Sequence
- Binding Sites
- Glutamic Acid
- Histidine
- Humans
- Hydro-Lyases
- Hydrogen-Ion Concentration
- Models, Chemical
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Pteridines
- Quinones
- Recombinant Proteins
- Structure-Activity Relationship
- Transcription Factors