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Article

Location of the active site and proposed catalytic mechanism of pterin-4a-carbinolamine dehydratase.

European journal of biochemistry - 1 Nov 1996

Köster S, Stier G, Ficner R, Hölzer M, Curtius H C, Suck D, Ghisla S

Abstract excerpt

Based on the recently solved three-dimensional structure of pterin-4a-carbinolamine dehydratase from rat/human liver the involvement of the proposed active-site residues Glu57, Asp60, His61, His62, Tyr69, His79, Arg87 and Asp88 was examined by site-directed mutagenesis. Most of the mutants showed reduced activity, and only the Glu57-->Ala mutant and the His61-->Ala, His62-->Ala double mutant were fully devoid of...

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PMID: 8944775
DOI: 10.1111/j.1432-1033.1996.00858.x

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