Article
Mutation of cysteine-295 to alanine in secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus affects the enantioselectivity and substrate specificity of ketone reductions.
Bioorganic & medicinal chemistry - 1 Jul 2001
Heiss C, Laivenieks M, Zeikus J G, Phillips R S
Abstract excerpt
The mutation of Cys-295 to alanine in Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase (SADH) was performed to give C295A SADH, on the basis of molecular modeling studies utilizing the X-ray crystal structure coordinates of the highly homologous T. brockii secondary alcohol dehydrog...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.