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Article

Complete reversal of coenzyme specificity by concerted mutation of three consecutive residues in alcohol dehydrogenase.

The Journal of biological chemistry - 17 Oct 2003

Rosell Albert, Valencia Eva, Ochoa Wendy F, Fita Ignacio, Parés Xavier, Farrés Jaume

Abstract excerpt

Gastric tissues from amphibian Rana perezi express the only vertebrate alcohol dehydrogenase (ADH8) that is specific for NADP(H) instead of NAD(H). In the crystallographic ADH8-NADP+ complex, a binding pocket for the extra phosphate group of coenzyme is formed by ADH8-specific residues Gly223-Thr224-His225, and the highly conserved Leu200 and Lys228. To investigate the minimal structural determinants for coenzyme...

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Identifiers

PMID: 12902331
DOI: 10.1074/jbc.M307384200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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