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Article

Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase.

Biochemistry - 8 Jan 2002

Numao Shin, Maurus Robert, Sidhu Gary, Wang Yili, Overall Christopher M, Brayer Gary D, Withers Stephen G

Abstract excerpt

Human pancreatic alpha-amylase (HPA) is a member of the alpha-amylase family involved in the degradation of starch. Some members of this family, including HPA, require chloride for maximal activity. To determine the mechanism of chloride activation, a series of mutants (R195A, R195Q, N298S, R337A, and R337Q) were made in which residues in the chloride ion binding site were replaced. Mutations in this binding site...

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Identifiers

PMID: 11772019
DOI: 10.1021/bi0115636

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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