Article
Crystal structures of three misacylating mutants of Escherichia coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP.
Biochemistry - 26 Nov 1996
Arnez J G, Steitz T A
Abstract excerpt
Three previously described mutant Escherichia coli glutaminyl-tRNA synthetase (GlnRS) proteins that incorrectly aminoacylate the amber suppressor derived from tRNATyr (supF) with glutamine were cocrystallized with wild-type tRNAGln and their structures determined. In two of the mutant enzymes stu...
Topics
- Acylation
- Adenosine Triphosphate
- Base Sequence
- Crystallography, X-Ray
- Electrons
- Escherichia coli
- Glutamate-tRNA Ligase
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Conformation
- RNA, Bacterial
- RNA, Transfer, Gln
- Structure-Activity Relationship
- Substrate Specificity