Article
Two chitin synthase genes from Ustilago maydis.
Microbiology (Reading, England) - 1 Feb 1996
Xoconostle-Cázares Beatriz, León-Ramirez Claudia, Ruiz-Herrera José
Abstract excerpt
PCR was used to amplify fragments corresponding to CHS genes from Ustilago maydis, utilizing as primers oligonucleotides devised according to the conserved regions of fungal CHS genes. The PCR product was employed as a probe to screen a genomic library of the fungus. Two different CHS genes (Umchs1 and Umchs2) were thus identified in the positive clones recovered. Their sequence revealed high similarity with the...
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