Article
The Candida albicans CHS4 gene complements a Saccharomyces cerevisiae skt5/chs4 mutation and is involved in chitin biosynthesis.
Microbiology (Reading, England) - 1 Jul 1999
Sudoh Masayuki, Tatsuno Kenji, Ono Naomi, Ohta Atsunori, Chibana Hiroji, Yamada-Okabe Hisafumi, Arisawa Mikio
Abstract excerpt
The Candida albicans CHS4 gene encoding chitin synthase 4 has been isolated using the Saccharomyces cerevisiae CHS4/SKT5 gene as a probe. The gene contains a 2061 bp open reading frame capable of encoding a protein of 687 amino acids (76053 Da). No intron was observed in the gene. Disruption of CHS4 in C. albicans yielded a Calcofluor-resistant phenotype, indicating that Chs4p contributes to chitin biosynthesis....
Topics
- Amino Acid Sequence
- Benzenesulfonates
- Blotting, Southern
- Candida albicans
- Cell Wall
- Chitin
- Chitin Synthase
- Chromosome Mapping
- Cloning, Molecular
- Genes, Fungal
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation