Article
Loss of virulence in Ustilago maydis by Umchs6 gene disruption.
Research in microbiology - 1 Mar 2004
Garcerá-Teruel Ana, Xoconostle-Cázares Beatriz, Rosas-Quijano Raymundo, Ortiz Lucila, León-Ramírez Claudia, Specht Charles A, Sentandreu Rafael, Ruiz-Herrera José
Abstract excerpt
A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by substitution of a central fragment of the Umchs6 gene with the hygromycin resistance cassette,...
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