Article
An investigation of the binding of protein proteinase inhibitors to trypsin by electrospray ionization mass spectrometry.
FEBS letters - 28 Oct 1996
Kraunsoe J A, Aplin R T, Green B, Lowe G
Abstract excerpt
The binding of BPTI and SBTI with trypsin has been investigated by ESI MS, using the mutant K15V-BPTI and the chemically modified RcamBPTI as controls. Although high cone voltages (+80 V) produce sharp spectra of BPTI, RcamBPTI, SBTI and trypsin alone, the complexes of BPTI, RcamBPTI and SBTI with trypsin undergo partial dissociation due to collisional activation. At lower cone voltages (+40 V) these non-covalent...
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