Article
Tandem mass spectrometry of protein-protein complexes: cytochrome c-cytochrome b5.
Journal of the American Society for Mass Spectrometry - 1 Jan 2002
Mauk M R, Mauk A G, Chen Yu-Luan, Douglas D J
Abstract excerpt
An improved method to interpret triple quadrupole MS/MS experiments of complexes of large ions is presented and applied to a study of the complex formed by the proteins cytochrome c and cytochrome b5. Modeling of the activation and dissociation process shows that most of the reaction occurs near the collision cell exit where ions have the highest internal energies. Experiments at different collision cell...
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