Article
Advantages and drawbacks of nanospray for studying noncovalent protein-DNA complexes by mass spectrometry.
Rapid communications in mass spectrometry : RCM - 1 Jan 2002
Gabelica Valérie, Vreuls Christelle, Filée Patrice, Duval Valérie, Joris Bernard, Pauw Edwin De
Abstract excerpt
The noncovalent complexes between the BlaI protein dimer (wild-type and GM2 mutant) and its double-stranded DNA operator were studied by nanospray mass spectrometry and tandem mass spectrometry (MS/MS). Reproducibility problems in the nanospray single-stage mass spectra are emphasized. The relative intensities depend greatly on the shape of the capillary tip and on the capillary-cone distance. This results in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.