Article
An assessment of the effects of cadaver donor bone marrow on kidney allograft recipient blood cell chimerism by a novel technique combining PCR and flow cytometry.
Transplantation - 27 Oct 1996
Garcia-Morales R, Esquenazi V, Zucker K, Gomez C I, Fuller L, Carreno M, Cirocco R, Alamo A, Karatzas T, Burke G W, Ciancio G, Temple D, Fernandez H, Ricordi C, Tzakis A, Miller J
Abstract excerpt
A new technique, the PCR-flow assay is described that has allowed for the serial identification and quantitation of discrete mononuclear cell subsets of donor (or recipient) bone marrow derived cells in cadaver kidney transplant recipients infused postoperatively with donor vertebral body bone marrow cells. With fixed permeabilized cells in flow cytometry the amplification power of the polymerase chain reaction...
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