Article
CFTR expression in C127 cells is associated with enhanced cell shrinkage and ATP extrusion in Cl(-)-free medium.
Biochemical and biophysical research communications - 23 Oct 1996
Rotoli B M, Bussolati O, Dall' Asta V, Hoffmann E K, Cabrini G, Gazzola G C
Abstract excerpt
In this study we have employed three lines of C127 murine cells. C127 CFTR w/t, C127 CFTR delta F508 and C127 mock, transfected with, respectively, wild type, delta F508 mutant human CFTR cDNA or the vector only. In the first 10 minutes of a Cl(-)-free incubation the three cell lines exhibit a si...
Topics
- Adenosine Triphosphate
- Cell Size
- Chlorides
- Cloning, Molecular
- Culture Media
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA, Complementary
- Humans
- Mutation
- Osmolar Concentration
- Tumor Cells, Cultured