Crystallization and preliminary X-ray diffraction studies of the Lb proteinase from foot-and-mouth disease virus.

doi:10.1002/pro.5560050921

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Article

Crystallization and preliminary X-ray diffraction studies of the Lb proteinase from foot-and-mouth disease virus.

Protein science : a publication of the Protein Society - 1 Sept 1996

Guarné A, Kirchweger R, Verdaguer N, Liebig H D, Blaas D, Skern T, Fita I

Abstract excerpt

Different crystal forms of the C23A mutant from the leader proteinase of foot-and-mouth disease virus were obtained by the hanging drop vapor diffusion technique, using MgCl2 and PEG 6000 as precipitants. Well-developed crystals, with cubic morphology growing to approximately 1.0 mm3 in size, pre...

Topics

Aphthovirus
Crystallization
Endopeptidases
Magnesium Chloride
Mutation
Osmolar Concentration
Polyethylene Glycols
Recombinant Proteins
Temperature
X-Ray Diffraction

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