Article
Crystallization and preliminary X-ray crystallographic analysis of the Rv2002 gene product from Mycobacterium tuberculosis, a beta-ketoacyl carrier protein reductase homologue.
Acta crystallographica. Section D, Biological crystallography - 1 Feb 2002
Yang Jin Kuk, Yoon Hye-Jin, Ahn Hyung Jun, Lee Byung Il, Cho Sang Hyun, Waldo Geoffrey S, Park Min S, Suh Se Won
Abstract excerpt
A 260-residue protein (FabG3) encoded by the Rv2002 gene of Mycobacterium tuberculosis shows amino-acid sequence similarity to beta-ketoacyl carrier protein (ACP) reductase, FabG. A soluble mutant (I6T/V47M/T69M) was produced by the green fluorescent protein-based directed-evolution method. It was crystallized at 296 K using the hanging-drop vapour-diffusion method. The diffraction quality of the crystal improved...
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