Crystallisation of wild-type and variant forms of a recombinant plant enzyme β-D-glucan glucohydrolase from barley (Hordeum vulgare L.) and preliminary X-ray analysis.

doi:10.3390/ijms11072759

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Article

Crystallisation of wild-type and variant forms of a recombinant plant enzyme β-D-glucan glucohydrolase from barley (Hordeum vulgare L.) and preliminary X-ray analysis.

International journal of molecular sciences - 19 Jul 2010

Luang Sukanya, Ketudat Cairns James R, Streltsov Victor A, Hrmova Maria

Abstract excerpt

Wild-type and variant crystals of a recombinant enzyme beta-d-glucan glucohydrolase from barley (Hordeum vulgare L.) were obtained by macroseeding and cross-seeding with microcrystals obtained from native plant protein. Crystals grew to dimensions of up to 500 x 250 x 375 mum at 277 K in the hanging-drops by vapour-diffusion. Further, the conditions are described that yielded the wild-type crystals with...

Topics

Crystallization
Crystallography, X-Ray
Glucosidases
Hordeum
Mutation
Recombinant Proteins
X-Ray Diffraction

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Identifiers

PMID: 20717535
DOI: 10.3390/ijms11072759
PMCID: PMC2920565

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