Article
Purification, crystallization and preliminary X-ray diffraction analysis of the catalytic domain of adenylyl cyclase Rv1625c from Mycobacterium tuberculosis.
Acta crystallographica. Section D, Biological crystallography - 1 Feb 2004
Ketkar Amit D, Shenoy Avinash R, Kesavulu Muppuru M, Visweswariah Sandhya S, Suguna Kaza
Abstract excerpt
The Rv1625c gene product is an adenylyl cyclase identified in the genome of Mycobacterium tuberculosis strain H37Rv. It shows sequence similarity to the mammalian nucleotide cyclases and functions as a homodimer, with two substrate-binding sites at the dimer interface. A mutant form of the catalytic domain of this enzyme, K296E/F363R/D365C (KFD-->ERC), was overexpressed in Escherichia coli cells in a soluble...
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