Article
Epigenetic factors controlling the development of avian Purkinje neurons.
Journal of neuroscience methods - 1 Aug 1996
Jeffrey P L, Meaney J, Tolhurst O, Weinberger R P
Abstract excerpt
Using a unique protocol, we have developed an avian neuron culture system in which a high yield of Purkinje neurons is obtained more readily than with pre-existing methods. Purkinje neurons were identified in vitro using the specific antibodies calbindin and cyclic GMP-kinase. Survival of Purkinje neurons was dependent on astrocyte contact and enhanced by astrocytic factors supplied to the medium by a monolayer...
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