Article
6-Coumarin diazonium salt: a specific affinity label of the Torpedo acetylcholinesterase peripheral site.
Molecular pharmacology - 1 Dec 1995
Schalk I, Ehret-Sabatier L, Le Feuvre Y, Bon S, Massoulie J, Goeldner M
Abstract excerpt
A 6-coumarin diazonium salt was synthesized and tested on Torpedo acetylcholinesterase as a site-directed irreversible probe for quaternary ammonium binding. The rate of the inactivation was examined as a function of time, inhibitor concentration, and pH, which allowed the determination of the dissociation and the rate constants of this efficient affinity labeling process. Protection experiments using...
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