Article
[Detection of mutations in the CFTR gene using the PCR-dependent preferential homoduplex formation assay (PCR-PHFA) system].
Nihon rinsho. Japanese journal of clinical medicine - 1 Feb 1996
Oka T, Nakano H, Yamane A
Abstract excerpt
A simple and efficient method for detecting DNA alterations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene has been developed using polymerase chain reaction dependent preferential homoduplex formation assay (PCR-PHFA). The technique is based on strand competition between a double labeled amplicon and an unlabeled amplicon during hybridization in a precisely controlled temperature...
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