Article
Comparison of heteroduplex and single-strand conformation analyses, followed by ethidium fluorescence visualization, for the detection of mutations in four human genes.
Molecular and cellular probes - 1 Jun 1995
Rossetti S, Corrà S, Biasi M O, Turco A E, Pignatti P F
Abstract excerpt
Non-isotopic DNA single-strand conformation analysis and heteroduplex analysis by ethidium bromide fluorescence visualization (SSCAE and HAE, respectively) were compared for the detection of 15 different naturally occurring mutations in 15 different DNA samples. The mutations included single nucl...
Topics
- Base Sequence
- Collagen
- Cystic Fibrosis Transmembrane Conductance Regulator
- Ethidium
- Fluorescence
- Hexosaminidase B
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Nephritis, Hereditary
- Nucleic Acid Heteroduplexes
- Osteogenesis Imperfecta
- Polymerase Chain Reaction