Article
Progressive activation of adult microglial cells in vitro.
Glia - 1 Mar 1996
Slepko N, Levi G
Abstract excerpt
Bulk-isolated microglial cells from the adult rat brain grown in N2 medium supplemented with 10% fetal calf serum survived for at least 2 weeks, and their purity was >99% at day 1 and >93% at day 7. The phenotype of freshly plated cells was comparable to that of "resting," ramified microglia in vivo. With time in culture and with different schedules, depending on the parameter considered, microglia acquired...
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