Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Modification of human neuron-specific enolase for application to radioimmunoassay.

Biochemistry and molecular biology international - 1 Jun 1996

Aoki T, Kaneta M, Onagi H, Morikawa J, Tsubota N, Watabe H

Abstract excerpt

A recombinant human neuron-specific enolase (R-NSE), isolated from Escherichia coli, could not be used in an RIA system because of instability upon labeling. To apply R-NSE to RIA and to simplify the purification procedure, the N- and C-terminals of R-NSE were modified by tyrosine- and histidine-tagging, respectively. SY-NSE, containing one additional tyrosine residue, was obtained from both soluble and insoluble...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Cloning, Molecular
Share to Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Molecular Sequence Data

Share to Mutation

Share to Plasmids

Share to Recombinant Proteins

Source

Identifiers

PMID: 8828797
DOI: 10.1080/15216549600201511

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Modification of human neuron-specific enolase for application to radioimmunoassay.PMID 8828797Read ↗

Sequential protection-modification method for selective sulfhydryl group derivatization in proteins having more than one cysteine.Semantic proximity Read ↗

Anti-alpha enolase multi-antibody specificity in human diseases. Clinical significance and molecular mechanismsSemantic proximity Read ↗

Insulin receptor antibody-sulfamidase fusion protein penetrates the primate blood-brain barrier and reduces glycosoaminoglycans in Sanfilippo type A cells.Semantic proximity Read ↗

Cloning and expression of neuron-specific enolase in the corpus luteum of dairy goats.Semantic proximity Read ↗

Pituitary Autoantibodies in Lymphocytic Hypophysitis Target Both γ- and a-Enolase – A Link with Pregnancy?Semantic proximity Read ↗

In vitro expression systems for the Huntington protein.Semantic proximity Read ↗

Hierarchy of Post-translational Modifications Involved in the Circulatory Longevity of GlycoproteinsSemantic proximity Read ↗

Alteration of substrate specificity of rat neurolysin from matrix metalloproteinase-2/9-type to -3-type specificity by comprehensive mutation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.