Article
Determination of the Neurospora crassa CYS 3 sulfur regulatory protein consensus DNA-binding site: amino-acid substitutions in the CYS3 bZIP domain that alter DNA-binding specificity.
Current genetics - 1 Sept 1996
Li Q, Marzluf G A
Abstract excerpt
CYS3 is the positive-acting global regulatory protein involved in the sulfur control circuit in Neurospora crassa and belongs to the family of bZIP DNA-binding proteins. Here we report a characterization of native DNA-binding sites recognized by CYS3. DNA footprinting experiments and systematic m...
Topics
- Amino Acid Sequence
- Conserved Sequence
- Cystathionine gamma-Lyase
- DNA, Fungal
- DNA-Binding Proteins
- Mutagenesis, Site-Directed
- Mutation
- Neurospora crassa
- Phenylalanine
- Saccharomyces cerevisiae Proteins
- Serine
- Transcription Factors
- Tyrosine