Article
Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain.
Molecular and cellular biology - 1 Dec 1991
Minehart P L, Magasanik B
Abstract excerpt
The GLN3 gene of Saccharomyces cerevisiae is required for the activation of transcription of a number of genes in response to the replacement of glutamine by glutamate as source of nitrogen. We cloned the GLN3 gene and constructed null alleles by gene disruption. GLN3 is not essential for growth, but increased copies of GLN3 lead to a drastic decrease in growth rate. The complete nucleotide sequence of the GLN3...
Topics
- Alleles
- Amino Acid Sequence
- Antibodies, Fungal
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cloning, Molecular
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation, Fungal