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Article

Reliable genotyping of samples with very low DNA quantities using PCR.

Nucleic acids research - 15 Aug 1996

Taberlet P, Griffin S, Goossens B, Questiau S, Manceau V, Escaravage N, Waits L P, Bouvet J

Abstract excerpt

Our purpose was to identify an experimental procedure using PCR that provides a reliable genotype at a microsatellite locus using only a few picograms of template DNA. Under these circumstances, it is possible (i) that one allele of a heterozygous individual will not be detected and (ii) that PCR-generated alleles or 'false alleles' will arise. A mathematical model has been developed to account for stochastic...

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Identifiers

PMID: 8774899
DOI: 10.1093/nar/24.16.3189
PMCID: PMC146079

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