Article
Determining microsatellite genotyping reliability and mutation detection ability: an approach using small-pool PCR from sperm DNA.
Molecular genetics and genomics : MGG - 1 Jan 2011
Macdonald Anna J, Sarre Stephen D, Fitzsimmons Nancy N, Aitken Nicola
Abstract excerpt
Microsatellite genotyping from trace DNA is now common in fields as diverse as medicine, forensics and wildlife genetics. Conversely, small-pool PCR (SP-PCR) has been used to investigate microsatellite mutation mechanisms in human DNA, but has had only limited application to non-human species. Trace DNA and SP-PCR studies share many challenges, including problems associated with allelic drop-out, false alleles...
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