Site-directed mutagenesis of Flaveria trinervia phosphoenolpyruvate carboxylase: Arg450 and Arg767 are essential for catalytic activity and Lys829 affects substrate binding.

doi:10.1016/0014-5793(96)00832-0

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Article

Site-directed mutagenesis of Flaveria trinervia phosphoenolpyruvate carboxylase: Arg450 and Arg767 are essential for catalytic activity and Lys829 affects substrate binding.

FEBS letters - 2 Sept 1996

Gao Y, Woo K C

Abstract excerpt

Phosphoenolpyruvate carboxylases (PEPC) of all known sequences contain 11 conserved arginine and two lysine residues located in highly conservative regions. Previous chemical modifications show that arginine and lysine residues are essential for catalytic activity. Three conserved residues, Arg45...

Topics

Amino Acid Sequence
Arginine
Base Sequence
Conserved Sequence
Glycine
Lysine
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Phosphoenolpyruvate Carboxylase
Plants

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